Primer

Part:BBa_K590084:Design

Designed by: Michael Brasino   Group: iGEM11_Washington   (2011-09-22)


pGA Prefix, reverse primer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 16
    Illegal BglII site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Does not contain Not1, which makes gibson cloning difficult.


Source

Synthetic

References